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Intern, Vector Biosciences

Company: Audentes Therapeutics, Inc.
Location: South San Francisco
Posted on: May 3, 2021

Job Description:

Why Work for Us

Are you ready to use your experience and expertise to make an impact on patients' lives? Audentes is a biotechnology company developing new genetic medicines for rare, life-threatening diseases. We are working urgently to progress our medicines and need additional team members to help us do so. Because our mission is so important, our values are to: Be BOLD: Find a Way, Care Deeply, and Get Stuff Done. If you'd like to be a part of this important mission, please apply to join our team.

About Audentes Therapeutics

Audentes Therapeutics, An Astellas Company, is developing genetic medicines for patients with rare, life-threatening diseases. Based on our innovative approach to developing genetic medicines, as well as our industry-leading internal manufacturing capability and expertise, we have become the Astellas Center of Excellence for their newly-created Genetic Regulation Focus Area. We are currently exploring three gene therapy modalities: gene replacement, exon skipping gene therapy, and vectorized RNA knockdown, with plans to expand our focus and geographic reach under Astellas. We are based in San Francisco, with manufacturing and laboratory facilities in South San Francisco and Sanford, North Carolina. For more information, please visit our website: www.audentestx.com

Our Values:

  • Be BOLD (Find a Way)
  • Care Deeply - for our patients, each other and our work
  • #GSD (Get Stuff Done)

What is the catalyst for you in your professional career? Are you ready to be a part of a bold, innovative company, to make a change and to make a difference? Listed below is a current opening for a smart, driven, experienced, and just plain nice individual who wants to join us and be a part of our journey.

The Role

The intern, Vector Biosciences will develop a method to detect the expression of Dystrophin in cells through either immunofluorescence or western blotting. The intern will have the opportunity to experience first-hand a research study from start to finish, including assay design and development, optimization, and ultimately application of the assay for vector screening and testing. Besides gaining exposure to day-to-day laboratory tasks, the intern will attend the DMD Research Project Team meetings and the Vector Biosciences team meetings, which are focused on discussing the scientific progress of the pre-clinical program projects at Audentes. Attendance at these meetings will allow the intern to learn about the scientific process and get a feel for what it's like to work in research in the biotechnology industry.

Primary Responsibilities

  • Culture and differentiate muscle cell lines; Key deliverables: confirm myotube cell differentiation based on morphological changes and pre-established assays
  • Develop immunofluorescence assay for Dystrophin expression; Key deliverables: use positive control cells to choose an appropriate antibody, set staining and acquisition parameters, use these parameters to test treated cells
  • Develop western blot assay for Dystrophin expression; Key deliverables: use positive control cell lysates and purified protein to choose an appropriate antibody and optimize protocol, then use this assay to test treated cells
  • Present data at team meetings; Key deliverables: prepare slides describing data with guidance from mentor, then present findings at team meetings.

About you

Must Have/Required

  • Pursuing a Bachelor's or graduate degree in Molecular/cell biology, biochemistry, biotechnology (any studies in Biomedical and Pharmaceutical Sciences)
  • Models our Core Values: Be Bold: Find a Way, Care Deeply, Get Stuff Done - is experienced as someone who exemplifies the culture we want to create; operates with transparency; is trusted

All qualified applicants will receive consideration for employment without regard to race, sex, color, religion, sexual orientation, gender identity, national origin, protected veteran status, or on the basis of disability.

Keywords: Audentes Therapeutics, Inc., South San Francisco , Intern, Vector Biosciences, Other , South San Francisco, California

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