Yoh Scientific is currently seeking a Research Associate II
(Mammalian Cell Culture, AKTA) for one of our top manufacturing
clients located in South San Francisco, CA. This Company is a
consistent leader in its field and is well known their services.
This is an advancement opportunity and all local candidates, who
meet the qualifications below, are encouraged to apply!
The successful candidate will be primarily responsible for the
mammalian expression (e.g. HEK293 cells) of integral membrane
protein reagents to support antibody, small molecule and research
Heterologous protein expression in mammalian cell culture with a
focus on transient transfection protocols.
Small scale expression and optimization screening to support
technology projects, in addition to scale-up expressions (2-6L) and
harvesting will be required depending on project needs.
Characterization of extracellular vesicles and expressed membrane
proteins using various biochemical methods will also be
SDS-PAGE, Western-blot analysis, ELISA, and endotoxin assays.
Protein purification and chromatography, operating AKTA
BS/MS degree with ~4-10 years of experience or a PhD with 0-2
years of experience in Cell Biology, Biochemistry, Structural
Biology or a related laboratory discipline.
Strong technical expertise in heterologous protein expression in
mammalian cell culture with a focus on transient transfection
Experience in a drug discovery or structural biology environment
are ideal Experience generating and characterizing stable cell
lines, particularly of multi-component targets, is desired.
Practical experience in optimizing small- and large-scale mammalian
cell expressions for improved protein titers and quality is highly
Familiarity with high-level recombinant membrane protein expression
or BacMam transduction methods Proficiency with methods to
characterize protein titer and quality is essential, including
SDS-PAGE, Western-blot analysis, ELISA, and endotoxin assays
Familiarity with protein purification and chromatography
techniques, including operating AKTA chromatography Experienced
performing parallel and/or high-throughput expression screening
with basic lab automation is a plus.
Self-motivated person with good communication and attention to
Team-oriented and collaborative nature.
Please e-mail your resume to firstname.lastname@example.org
Please then call/ text Phil at 860.405.7181
What’s In It For You?
We welcome you to be a part of one of the largest global
staffing companies to meet your career aspirations. Yoh’s network
of client companies has been employing professionals like you for
over 65 years in the U.S., UK and Canada. Join Yoh’s extensive
talent community that will provide you with access to Yoh’s vast
network of opportunities and gain access to this exclusive
opportunity available to you.
Medical, Dental & Vision Benefits 401K Retirement Saving Plan
Life & Disability Insurance Direct Deposit & weekly ePayroll
Employee Discount Programs Referral Bonus Programs
Yoh makes finding and applying for jobs simple. Partner with Yoh
to find the right opportunities across multiple industries in the
US and UK. Find out more here!
Yoh, a Day & Zimmermann company, is an Equal Opportunity
Employer. All qualified applicants will receive consideration for
employment without regard to race, color, religion, sex, sexual
orientation, gender identity, national origin, disability, or
status as a protected veteran. Visit
https://www.yoh.com/applicants-with-disabilities to contact us if
you are an individual with a disability and require accommodation
in the application process.