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Research Associate II (Mammalian Cell Culture, AKTA)

Company: Yoh
Location: South San Francisco
Posted on: June 4, 2021

Job Description:

Yoh Scientific is currently seeking a Research Associate II (Mammalian Cell Culture, AKTA) for one of our top manufacturing clients located in South San Francisco, CA. This Company is a consistent leader in its field and is well known their services. This is an advancement opportunity and all local candidates, who meet the qualifications below, are encouraged to apply!

DESCRIPTION:

DUTIES:

The successful candidate will be primarily responsible for the mammalian expression (e.g. HEK293 cells) of integral membrane protein reagents to support antibody, small molecule and research discovery projects.
Heterologous protein expression in mammalian cell culture with a focus on transient transfection protocols.
Small scale expression and optimization screening to support technology projects, in addition to scale-up expressions (2-6L) and harvesting will be required depending on project needs.
Characterization of extracellular vesicles and expressed membrane proteins using various biochemical methods will also be pursued.
SDS-PAGE, Western-blot analysis, ELISA, and endotoxin assays.
Protein purification and chromatography, operating AKTA chromatography systems.

REQUIREMENTS:

BS/MS degree with ~4-10 years of experience or a PhD with 0-2 years of experience in Cell Biology, Biochemistry, Structural Biology or a related laboratory discipline.
Strong technical expertise in heterologous protein expression in mammalian cell culture with a focus on transient transfection protocols.
Experience in a drug discovery or structural biology environment are ideal Experience generating and characterizing stable cell lines, particularly of multi-component targets, is desired.
Practical experience in optimizing small- and large-scale mammalian cell expressions for improved protein titers and quality is highly desired.
Familiarity with high-level recombinant membrane protein expression or BacMam transduction methods Proficiency with methods to characterize protein titer and quality is essential, including SDS-PAGE, Western-blot analysis, ELISA, and endotoxin assays etc.
Familiarity with protein purification and chromatography techniques, including operating AKTA chromatography Experienced performing parallel and/or high-throughput expression screening with basic lab automation is a plus.
Self-motivated person with good communication and attention to detail.
Team-oriented and collaborative nature.

Please e-mail your resume to philip.cooke@yoh.com

Please then call/ text Phil at 860.405.7181

What’s In It For You?

We welcome you to be a part of one of the largest global staffing companies to meet your career aspirations. Yoh’s network of client companies has been employing professionals like you for over 65 years in the U.S., UK and Canada. Join Yoh’s extensive talent community that will provide you with access to Yoh’s vast network of opportunities and gain access to this exclusive opportunity available to you.

Medical, Dental & Vision Benefits 401K Retirement Saving Plan Life & Disability Insurance Direct Deposit & weekly ePayroll Employee Discount Programs Referral Bonus Programs

Yoh makes finding and applying for jobs simple. Partner with Yoh to find the right opportunities across multiple industries in the US and UK. Find out more here!

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Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.

Keywords: Yoh, South San Francisco , Research Associate II (Mammalian Cell Culture, AKTA), Other , South San Francisco, California

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