Principal Scientific Researcher - Structural Biology Department
Location: South San Francisco
Posted on: June 7, 2021
The Department of Structural Biology at Genentech has an
exciting opportunity for a highly motivated Principal Scientific
Researcher (PSR) with expertise in protein biochemistry and
structural biology. This Principal Scientific Researcher will join
a group focused on generation and optimization of recombinant
proteins for drug discovery efforts and discovery research. Working
with multiple groups across Genentech, the Researcher will be
responsible for generating high-quality protein reagents to support
small and large molecule drug discovery, high throughput screening,
assay development and structural biology efforts. This role will
involve considerable bench work, including the use of robotic
platforms for protein purification, and protein characterization by
biophysical & biochemical methods. Hands-on experience with X-ray
crystallography, including sample preparation and crystallization
is highly desirable.The individual will work independently at the
bench and collaborate with colleagues across various functional
areas to generate and communicate data to further research and drug
The successful candidate must have either a BS/MS degree with
~6-10 years of experience or a PhD with 0-2 years of experience in
Biochemistry, Chemical Biology, Structural Biology or a related
discipline. Relevant experience in generating challenging proteins
across a range of protein families is required. A record of
achievement and collaboration, documented by peer-reviewed
publications is highly valued.
Additional Desired Qualifications:
Strong candidates will have many of the following skills and
Working knowledge of construct design strategies.
Experience in protein expression systems such as E coli,
mammalian or insect.
Hands-on experience designing and optimizing the production of
challenging protein targets for structural and/or functional
Experience in purification and assembly of multi-protein
Expertise with modern protein purification and chromatography
techniques, and knowledgeable with operating AKTA chromatography
systems as well as analytical HPLC systems.
Hands-on experience in protein crystallization, crystal
optimization and harvesting for data collection.
Exposure to cryo-EM sample and grid preparation.
Proficiency with methods to characterize protein quality and
purity, such as mass spectrometry, SEC-MALS/DLS, endotoxin assays,
CD, and DSF/thermal shift assays.
Expertise with biophysical and biochemical protein-protein,
protein-nucleic acid interaction techniques and/or functional
Passion for exploring and developing new technologies.
Excellent communication, interpersonal and organizational
- Team-oriented, collaborative nature with fearless attitude to
tackle long standing difficult problems in research and drug
Keywords: Genentech, South San Francisco , Principal Scientific Researcher - Structural Biology Department, Other , South San Francisco, California
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